Journal: The Journal of nutritional biochemistry

Article Title: Disruption of hepatic one-carbon metabolism impairs mitochondrial function and enhances macrophage activity in methionine–choline-deficient mice ☆☆ , ☆☆☆

doi: 10.1016/j.jnutbio.2020.108381

Figure Lengend Snippet: Adenosine receptor expression in livers of MCD and alloxazine-treated mice.Western blot images of ADORA1, ADORA2A, ADORA2B and ADORA3 in mouse liver (A). Relative expression of proteins measured by western blotting for ADORA1 (B), ADORA2A (C), ADORA2B (D) and ADORA3 (E). White bars represent CTR+Veh, light gray represent CTR+ALX, dark gray bars represent MCD+Veh, and dark bars represent MCD+ALX. Different lowercase letters denote a significant difference (P<.05, n=3). All data are presented as mean ± standard deviation.

Article Snippet: Antibodies (Supplementary Table S3) were obtained from Cell Signaling Technologies with the following exceptions: adenosine A 1 receptor (ADORA1) (Proteintech), adenosine A 2B receptor (ADORA2B) (Millipore), OXPHOS Total Complex (Abcam, UK), adenosine A 2A receptor (ADORA2A), adenosine A 3 receptor (ADORA3), serine hydroxymethyltransferase 1 (SHMT1), serine hydroxymethyltransferase 2 (SHMT2), and alpha tubulin, Vinculin (Fisher) and StarBright Blue 700 Goat Anti-Rabbit IgG (BioRad, Hercules, CA, USA).

Techniques: Expressing, Western Blot, Standard Deviation

Journal: The Korean Journal of Parasitology

Article Title: Adenosine A 3 Receptor Mediates ERK1/2- and JNK-Dependent TNF-α Production in Toxoplasma gondii -Infected HTR8/SVneo Human Extravillous Trophoblast Cells

doi: 10.3347/kjp.2020.58.4.393

Figure Lengend Snippet: Toxoplasma gondii infection increased TNF-α secretion and MAPK activation in HTR8/SVneo cells. HTR8/SVneo cells were infected with T. gondii at an MOI of 10 for the indicated time durations. (A) TNF-α secretion levels evaluated by ELISA. *** P <0.001 compared with mock-infected control cells. (B) Expression level of MAPK pathway molecules assessed by western blots analysis. α-Tubulin was used as a loading control.

Article Snippet: Primary antibodies against the following proteins were used: adenosine A 1 -R (A 1 AR), adenosine A 2A -R (A 2A AR), adenosine A 2B -R (A 2B AR), adenosine A 3 -R (A 3 AR), and α-tubulin (all from Santa Cruz Biotechnology); and phospho-p38 MAPK, p38 MAPK, phospho-ERK1/2, ERK1/2, phospho-JNK, and JNK (all from Cell Signaling Technology Inc., Danvers, Massachusetts, USA).

Techniques: Infection, Activation Assay, Enzyme-linked Immunosorbent Assay, Control, Expressing, Western Blot

Journal: The Korean Journal of Parasitology

Article Title: Adenosine A 3 Receptor Mediates ERK1/2- and JNK-Dependent TNF-α Production in Toxoplasma gondii -Infected HTR8/SVneo Human Extravillous Trophoblast Cells

doi: 10.3347/kjp.2020.58.4.393

Figure Lengend Snippet: Roles of A 3 AR in T. gondii -induced MAPK activation and TNF-α secretion in HTR8/SVneo cells. HTR8/SVneo cells were transfected with control siRNA or A 3 AR-specific siRNAs and infected with T. gondii at an MOI of 10 for 24 hr. (A) Expression level of A 3 AR and MAPK pathway molecules assessed by western cell culture supernatants blot α-Tubulin was used as a loading control. (B) Concentration of TNF-α in was evaluated by ELISA. (C) Inhibitory effect of p38 MAPK (SB203580), ERK1/2 (PD098059), and JNK (SP600125) on TNF-α secretion levels in T. gondii -infected cells. HTR8/SVneo cells were pretreated with or without 30 μM SB203580, PD098059 or SP600125 for 2 hr and subsequently infected with T. gondii at an MOI 10 of for 24 hr. TNF-α secretion levels was evaluated by ELISA. *** P <0.001 compared with respective control.

Article Snippet: Primary antibodies against the following proteins were used: adenosine A 1 -R (A 1 AR), adenosine A 2A -R (A 2A AR), adenosine A 2B -R (A 2B AR), adenosine A 3 -R (A 3 AR), and α-tubulin (all from Santa Cruz Biotechnology); and phospho-p38 MAPK, p38 MAPK, phospho-ERK1/2, ERK1/2, phospho-JNK, and JNK (all from Cell Signaling Technology Inc., Danvers, Massachusetts, USA).

Techniques: Activation Assay, Transfection, Control, Infection, Expressing, Western Blot, Cell Culture, Concentration Assay, Enzyme-linked Immunosorbent Assay